Stable gene expression for normalisation and single-sample scoring
نویسندگان
چکیده
منابع مشابه
Identifying Stable Reference Genes for qRT-PCR Normalisation in Gene Expression Studies of Narrow-Leafed Lupin (Lupinus angustifolius L.)
Quantitative Reverse Transcription PCR (qRT-PCR) is currently one of the most popular, high-throughput and sensitive technologies available for quantifying gene expression. Its accurate application depends heavily upon normalisation of gene-of-interest data with reference genes that are uniformly expressed under experimental conditions. The aim of this study was to provide the first validation ...
متن کاملSample size for gene expression microarray experiments
MOTIVATION Microarray experiments often involve hundreds or thousands of genes. In a typical experiment, only a fraction of genes are expected to be differentially expressed; in addition, the measured intensities among different genes may be correlated. Depending on the experimental objectives, sample size calculations can be based on one of the three specified measures: sensitivity, true disco...
متن کاملMulti-Dimensional Feature Scoring For Gene Expression Data
Motivation: The analysis of gene expression data presents researchers with the problem of finding optimal subsets of genes to focus on. This is a computational and statistical challenge, mostly due to the high-dimensionality of the data and the small amounts of samples. Hence, an initial process of gene (feature) selection is usually performed. Results: This paper discusses several methods that...
متن کاملUnconstrained versus constrained acoustic normalisation in confidence scoring
In HMM-based recognition systems for large vocabulary, the observation likelihoods provided by the acoustic models are useful in confidence measures if they are properly normalised. This paper compares two normalisation methods for the acoustic model likelihoods: unconstrained normalisation, based on the unconditional observation likelihood, and constrained normalisation, based on the observati...
متن کاملHigh throughput sample processing and automated scoring
The comet assay is a sensitive and versatile method for assessing DNA damage in cells. In the traditional version of the assay, there are many manual steps involved and few samples can be treated in one experiment. High throughput (HT) modifications have been developed during recent years, and they are reviewed and discussed. These modifications include accelerated scoring of comets; other impo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2020
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkaa802